Sample Requirements

454 GS FLX Roche

Sample Project Type Quantity Volume Quality
DNA Whole Genome Shotgun 1 µg ≤ 80 µl

Double stranded, not degraded, without particles and contamination A260/280 ≥ 1.8 Purification with column or gel (Recommended) Wide Orifice Tips (Paired Ends recommended projects)

Amplicon sequencing 1 µg ≤ 80 µl
Paired Ends 3Kb 5 µg ≤ 200 µl
Paired Ends 8Kb 30 µg ≤ 250 µl
Paired Ends 20Kb 60 µg ≤ 250 µl
Total RNA Transcriptómica-RNA Seq 80 µg ≤ 100 µl 2100 Bioanalyzer RNA Integrity Number> 8 (Recommended) Proporción 28S rRNA : 18S rRNA = 2:1 (Recommended) A260/280 ≥ 1.8 DNA free